期刊简介
本刊于2006年2月25日创刊,是中华人民共和国教育部主管、华中科技大学同济医学院主办的国家级医学类期刊,由中科院杨雄里院士、王永炎院士等担任名誉主编,由武汉同济医院神经内科主任王伟教授担任主编。本刊紧跟国际神经科学发展趋势,对神经系统疾病的基础与临床研究的新动态、新技术、新进展和新经验,予以实时追踪、报道,同时对基层医务工作者所关注的临床诊断方法、治疗方法、相关技能予以介绍,突出科学性、创新性和实用性。本刊坚持理论与实践相结合,普及与提高相结合,国内与国外相结合,交流神经病学领域的学术研究成果和实践经验,促进我国神经病学事业的蓬勃发展。欢迎广大神经内外科、内科等科室的临床医师、各大科研机构的研究人员、进修生、研究生、博士生以及医学院校的教师等踊跃投稿、订阅。
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首页>神经损伤与功能重建杂志

- 杂志名称:神经损伤与功能重建杂志
- 主管单位:中华人民共和国教育部
- 主办单位:华中科技大学同济医学院
- 国际刊号:1001-117X
- 国内刊号:42-1759/R
- 出版周期:双月刊
期刊荣誉:1998年获湖北省优秀期刊期刊收录:统计源核心期刊(中国科技论文核心期刊), 国家图书馆馆藏, 知网收录(中), 维普收录(中), 上海图书馆馆藏, 万方收录(中)
Effect of Cell Cycle Inhibitor Olomoucine on Astroglial Proliferation and Scar Formation after Focal Cerebral Infarction in Rats
关键词:proliferation, astrocytic scar, cell cycle, Cyclins, cyclin dependent kinase, Olomoucine, Cerebral Infarction
摘要:Background: Astrocytes become reactive following many types of CNS injuries.Excessive astrogliosis is detrimental and contributes to neuronal damage. We sought to determine whether inhibition of cell cycle could decrease the proliferation of astroglial cells and therefore reduce excessive gliosis and glial scar formation after focal ischemia. Methods: Cerebral infarctionmodel was induced by photothrombosis method. Rats were examined using MRI, and lesion volumes were estimated on day 3 post-infarction. The expression of glial fibrillary acidic protein(GFAP) and proliferating cell nuclear antigen(PCNA) was observed by immunofluorescence staining. Protein levels for GFAP, PCNA, Cyclin A and Cyclin B1 were determined by Western blot analysis from the ischemic and sham animals sacrificed at 3,7,30 days after operation. Results:Cell cycle inhibitor olomoucine significantly suppressed GFAP and PCNA expression and reduced lesion volume after cerebral ischemia. In parallel studies, we found dense astroglial scar in boundary zone of vehicle-treated rats at 7 and 30 days. Olomoucine can markedly attenuate astroglial scar formation. Western blot analysis showed increased protein levels of GFAP, PCNA,Cyclin A and Cyclin B1 after ischemia, which was reduced by olomoucine treatment. Conclusion:Our results suggested that astroglial activation, proliferation and subsequently astroglial scar formation could be partially inhibited by regulation of cell cycle. Cell cycle modulation thereby pro-vides a potential promising strategy to treat cerebral ischemia.
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